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. 2020 Feb 5;11:722. doi: 10.1038/s41467-019-14172-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Day 0, 3, 7, and 21 combined canonical correlation analysis and T-distributed stochastic neighbor embedding (t-SNE) plot identified 12 distinct cell clusters based on gene expression differences. b Violin plots of monocyte/macrophage markers (Siglec1, Arg1, Mrc1, Csfr1), Tgfb1, and Tgfbr1. c t-SNE plot displaying only those cells in the canonical correlation analysis from day 0. d Feature plots of monocyte/macrophage genes expressed to identify possible resident macrophages at the extremity injury site from day 0 cells.