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. 2020 Jan 30;13:7. doi: 10.3389/fnmol.2020.00007

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Copyright © 2020 Xu, Zheng, Yin, Xu, Tian, Zhang, Wang and Ma.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Experimental design. Mice (normal training, NT: n = 30, overtraining, OT: n = 30) exercised according to the training program, including a treadmill training protocol and two incremental load tests (ILT). During the second stage of the treadmill running protocol, the NT group ran at Grade = −14%, Speed = 60% EV and Time = 60 min. Forty-eight hours after the end of the last ILT, the mice were killed to take tissue samples (NT: n = 15, OT: n = 15) or perform Morris water maze experiments (NT: n = 15, OT: n = 15), respectively.