FIGURE 3.

Partial least squares projection to latent structures (PLS) analysis for γ-PGA producing strains based on a promoter library. The analysis was carried out for the non-γ-PGA producing reference (WT) and strains with newly generated promoters PV35.1, PV35.3, and PV35.26 controlling pgs expression. Biological triplicates for each strain were cultivated in glucose minimal medium. Samples for analysis were taken during the exponential phase (9 h). The separation according to the metabolom analysis (A), the observed versus predicted γ-PGA values (B),the VIP values (C), and loading values (D) for metabolites highly connected to γ-PGA production are shown. The loading data for metabolites with loading values >0.1 are shown whereas negative values correspond to metabolites decreasing with increasing γ-PGA production and metabolites with positive values are increased for higher γ-PGA. ADP, adenosine 5′-diphosphate; AICAR 5′-phosphoribosyl-5-amino-4-imidazolecarboxamide; AMP, adenosine 5′-monophosphate; DHAP, dihydroxyacetone phosphate; G6P, glucose-6-phosphate; KDPG, 2-Keto-3-deoxy-6-phosphogluconate; IMP, Inosine 5′-monophosphate; MEP, methylerythritol 4-phosphate; PRPP, 5-phospho-alpha-D-ribose 1-diphosphate; R1P, ribose-1-phosphate; R5P, ribose-5-phosphate; SBP, sedoheptulose 1,7-bisphosphate; UDP-Glc, uridine 5′-diphosphate-glucose; XMP, xanthosine 5′-phosphate.