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. 2020 Feb 5;10:1860. doi: 10.1038/s41598-020-58041-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) A schematic of the assay format. Anti-alemtuzumab antibodies present in serum bind to the Alem Globody. The total IgG is captured on Protein G allowing detection of the retained luciferase. In panel (b) in the absence of ADA the Globody is not retained. To determine the specificity of the assay, (c) Alem GloBody was incubated with ADA against alemtuzumab, adalimumab, ustekinumab and trastuzumab. Luciferase signal was only detected with ADA against alemtuzumab. (d) Conversely, Adali GloBody was incubated with ADA against alemtuzumab, adalimumab, ustekinumab and trastuzumab. Luciferase signal was detected with ADA against adalimumab, but no luciferase signal was detected with ADA against alemtuzumab, ustekinumab or trastuzumab.