Fig. 2.

Flow cytometric analysis of mesenchymal stem cell (MSC) makers. MSC isolated from two different sources. MSC were harvested at passage 3–5, labeled with monoclonal antibodies against CD14, CD31, CD34, CD45-, CD44, CD73, CD90-, CD105, HLA-DR, and analyzed by flow cytometry. One representative experiment is reported on AT-MSC and OE-MSC that obtained after the third passage. Filled red histogram shows negative control and blue line displays indicated marker. For flow cytometric analysis of MSC surface markers (CD44, CD73, CD90, and CD105) that have expression more than 75%, unstained cell was used as negative control. However, for analysis of CD14, CD31, CD34, CD45, and HLA-DR markers that have low expression, isotype control was used as negative control. Meanwhile, unspecific binding was removed by three time washing and by FC receptor blacking. (Color figure online)