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. 2020 Jan 2;72(1):57–68. doi: 10.1007/s10616-019-00357-8

Table 1.

Forward and reverse primers of GAPDH, TLR3, TLR4, Nestin, Vimentin and N-cadherin genes for real-time PCR amplification

Gene name Primers Sequences Product size (bp)
TLR3 Forward 5′-GCTGCAGTCAGCAACTTCAT-3′ 144
Reverse 5′-AGGAAAGGCTAGCAGTCATCC-3′
TLR4 Forward 5′-GATGAGGACTGGGTAAGGAATG-3′ 102
Reverse 5′-GGCCACACCGGGAATAAA-3′
Nestin Forward 5′-GTAGCTCCCAGAGAGGGGAA-3′ 206
Reverse 5′-CTCTAGAGGGCCAGGGACTT-3′
Vimentin Forward 5′-GGTGGACCAGCTAACCAACGA-3′ 183
Reverse 5′-TCAAGGTCAAGACGTGCCAG-3′
N-Cadherin Forward 5′-GGACAGTTCCTGAGGGATCA-3′ 253
Reverse 5′-GGATTGCCTTCCATGTCTGT-3′
GAPDH Forward 5′-GCACCGTCAAGGCTGAGAAC-3′ 138
Reverse 5′-TGGTGAAGACGCCAGTGGA-3′