Table 2.
Primers | Sequences |
---|---|
kan-CF kan-CR gfp-CF gfp-CR kan-AF kan-AR |
5′-CCCAAGCTTAAGGAGATATACATATGATTGAACAAGATGGATTGC-3′ 5′-ACGCGTCGACTCAGAAGAACTCGTCAAGAAGGCGATAGA-3′ 5′-ACGCGTCGACAAGGAGATATACATATGCGTAAAGGCGAAGAGCTG-3′ 5′-CGCGGATCCTCATTTGTACAGTTCATCCATACCAT-3′ 5′-CCGGAATTCAAGGAGCATCTATGATTGAACAAGATGGATTGCACGCAGGT-3′ 5′-CGCGGATCCTCAGAAGAACTCGTCAAGAAGGCGATAGAA-3′ |
gfp-AF | 5′-CGCGGATCCAAGGAGCATCTATGCGTAAAGGAGAAGAACT-3′ |
gfp-AR | 5′-CGCGGATCCTCATTTGTACAGTTCATCCATACCAT-3′ |
Primers for qRT-PCR | |
rrsG-QF | GGTGTAGCGGTGAAATGCGTAG |
rrsG-QR | TCAAGGGCACAACCTCCAAGTC |
kanr-F | CTGCTATTGGGCGAAGTG |
kanr-R | GATGTTTCGCTTGGTGGTC |
gfp-QF | TCACTACTTTCGGTTATGG |
gfp-QF | AAACTTGACTTCAGCACG |