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. 2020 Feb 5;10(3):93. doi: 10.1007/s13205-020-2100-2

Table 2.

Primers used in this study

Primers Sequences

kan-CF

kan-CR

gfp-CF

gfp-CR

kan-AF

kan-AR

5′-CCCAAGCTTAAGGAGATATACATATGATTGAACAAGATGGATTGC-3′

5′-ACGCGTCGACTCAGAAGAACTCGTCAAGAAGGCGATAGA-3′

5′-ACGCGTCGACAAGGAGATATACATATGCGTAAAGGCGAAGAGCTG-3′

5′-CGCGGATCCTCATTTGTACAGTTCATCCATACCAT-3′

5′-CCGGAATTCAAGGAGCATCTATGATTGAACAAGATGGATTGCACGCAGGT-3′

5′-CGCGGATCCTCAGAAGAACTCGTCAAGAAGGCGATAGAA-3′

gfp-AF 5′-CGCGGATCCAAGGAGCATCTATGCGTAAAGGAGAAGAACT-3′
gfp-AR 5′-CGCGGATCCTCATTTGTACAGTTCATCCATACCAT-3′
Primers for qRT-PCR
rrsG-QF GGTGTAGCGGTGAAATGCGTAG
rrsG-QR TCAAGGGCACAACCTCCAAGTC
kanr-F CTGCTATTGGGCGAAGTG
kanr-R GATGTTTCGCTTGGTGGTC
gfp-QF TCACTACTTTCGGTTATGG
gfp-QF AAACTTGACTTCAGCACG