Table 2.
Gene expression in MDCK cells
| Gene | Expression | ∆Ct ± SD |
|---|---|---|
| GAPDH | + | HK |
| HPRT1 | + | 3.6 ± 0.2 |
| ACTB | + | − 1.81 ± 0.6 |
| B2M | + | 0.74 ± 0.4 |
| IL-1β | ± | 19.4 ± 1.5 |
| IL-2 | ± | 20.0 ± 1.2 |
| IL-4 | − | NA |
| IL-5 | + | 14.6 ± 0.5 |
| IL-6 | + | 15.8 ± 0.6 |
| IL-8 | + | 8.5 ± 0.7 |
| IL-10 | − | NA |
| IL-12 | ± | 21.2 ± 0.7 |
| IL-15 | − | NA |
| IL-16 | + | 14.2 ± 0.5 |
| IL-17 | − | NA |
| IL-18 | + | 16.4 ± 0.7 |
| IL-23 | + | 10.8 ± 0.7 |
| IL-27 | − | NA |
| TNF-α | ± | 20.6 ± 0.7 |
| IFN-γ | − | NA |
| TLR1 | + | 14.2 ± 0.5 |
| TLR2 | ± | 19.4 ± 1.5 |
| TLR3 | + | 9.0 ± 0.6 |
| TLR4 | ± | 18.4 ± 0.6 |
| TLR5 | + | 15.8 ± 0.7 |
| TLR7 | ± | 20.8 ± 2.1 |
| TLR8 | ± | 21.0 ± 2.3 |
| TLR9 | ± | 20.8 ± 1.3 |
| TLR10 | ± | 20.6 ± 1.8 |
| p53 | + | 12.6 ± 1.1 |
| CD14 | + | 10.5 ± 1.1 |
| MD2 | + | 7.0 ± 0.6 |
| Myd88 | + | 6.6 ± 1.2 |
| NF-kB/p65 | + | 6.3 ± 0.7 |
| STAT-5a | + | 14.2 ± 0.5 |
| iNOS | + | 9.8 ± 0.8 |
| CD44 | + | 4.3 ± 0.7 |
| CXCR4 | + | 13.2 ± 1.3 |
| RAD51 | + | 6.1 ± 0.8 |
| PTEN | + | 18.4 ± 1.3 |
| ErbB2 | + | 10.0 ± 1.4 |
Data are expressed as: + all samples were positive; − no positive samples were detected; ± only some samples tested positive. GAPDH was shown to be most stable, and hence chosen to calculate the ΔCt values
NA not available, HK housekeeping gene