Fig. 3.

6-Ketocholestanol alters lipogenic gene expressions in HepG2 cells. HepG2 cells were treated with ethanol (vehicle control) or 7 μM 6-keto for 6 h. The expression levels of each gene (NR1H3, ABCA1, SREBF1, FASN, and SCD) were normalized to the TUBB2A expression level. The expression level of each gene in the vehicle control was arbitrarily set at 1. Data are presented as the mean ± SEM of three independent experiments. Columns marked with asterisk(s) are significantly different from the control (*P < 0.05, **P < 0.01), analyzed using the Student’s t-test. ABCA1 ATP binding cassette subfamily A member 1, FASN fatty acid synthase, NR1H3 nuclear receptor subfamily 1 group H member 3 (encoding liver X receptor α), SCD stearoyl-CoA desaturase (encoding SCD1), SREBF1 sterol regulatory element binding transcription factor 1 (encoding sterol regulatory element-binding protein-1c)