Fig. 7.

An increase in human FASN gene promotor activity by TO901317 is inhibited by the addition of 6-ketocholestanol. After transfection, HepG2 cells were treated with ethanol (the vehicle control), 1 μM TO, 7 μM 6-keto, or 1 μM TO + 7 μM 6-Keto for 24 h. Each luciferase activity of the vehicle control was arbitrarily set at 1. Data are presented as the mean ± SEM of three independent experiments. Columns marked with different letters are significantly different at P < 0.05 by one-way ANOVA followed by the Tukey–Kramer multiple comparison post hoc test