Fig. 8.

6-Ketocholestanol, 6α-hydroxycholestanol, and 6β-hydroxycholestanol, oxidized in the 6-carbon of B-ring, suppress human FASN gene promoter activities. After transfection, HepG2 cells were treated with ethanol (the vehicle control), 7 μM 7α-OH, 7 μM 7β-OH, 7 μM 6α-OH, 7 μM 6β-OH, or 7 μM 6-Keto for 24 h. Data are presented as the mean ± SEM of three independent experiments. Columns marked with asterisk(s) are significantly different from the control (*P < 0.05, **P < 0.01) by one-way ANOVA followed by the Dunnett’s multiple comparison post hoc test