Study of HIF-1α expression in the parental and CRR cells by western blot analysis. Parental (HepG2, HeLa, and SAS) and CRR cells (HepG2-8960-R, HeLa-R, and SAS-R) were underwent 10-Gy irradiation followed by evaluation of HIF-1α expression at different time points. Expression of HIF-1α was stabilized in CRR cells after 12 h while in the parental cells was decreased at this time. β-actin was also detected as loading a control. NIOR the cells with no irradiation, h hours, D days, IR irradiation