Table 1.

Summary of microbiological profile of scrapes carried out between 4th April 2006 and 31st October 2017 at Bristol Eye Hospital and Royal United Hospital, Bath, separated according to years to assess for trends over time

	2006–2011	2012–2017	P value*
	(Total organisms N = 550)	(Total organisms N = 645)
Total scrapes	1187	1427
Total positive scrapesa	454	542	0.89
Percentage culture positive	38.24%	37.98%	0.89
Gram-positive total	339	421	0.193
Streptococci	39	45	0.9387
S. aureus	33	48	0.323
CoNS	193	237	0.5528
Bacilli	20	20	0.6079
Othersb	54	71	0.503
Gram-negative total	165	170	0.1622
Pseudomonas spp.	94	95	0.2647
Moraxella sppc	15	22	0.4965
Othersd	56	53	0.2397
Fungi	41	42	0.5228
Acanthamoeba sppe	5	12	0.1663

^aNote the total number of organisms is greater than the total positive scrape number, as this includes samples that cultured multiple organisms

^bGram-positive other includes Enterococci, Micrococcus spp, diphtheroids

^cMoraxella species include M. catarrhalis, M. lacunata, M. nonliquefaciens, Moraxella spp. (MOR)

^dGram-negative other includes Enterobacter spp, Citrobacter spp, Escherichia spp, Haemophilus influenzae, Klebsiella spp, Morganella spp, Proteus spp and Serratia spp

^eNote during the study period clinically that there were a greater number of acanthamoeba keratitis (AK) cases seen. Other methods including PCR sent to a reference laboratory and confocal microscopy were used for the diagnosis of these cases and these scrape results may not therefore provide a true representation of the number of AK cases seen

*p value calculated using the chi-squared test. No statistically significant difference identified between the two time frames and organisms seen, or in the percentage of culture-positive scrapes