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. 2020 Feb 5;10:1856. doi: 10.1038/s41598-020-58776-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Western blot analyses of tomato leaf stress-related protein profiles upon treatment with the pharmaceutical cocktail at 100 ppb. Roots of tomato seedlings were bathing in tap water (−) and in a cocktail of Carbamazepine, Valporic acid, Phenytoin, Diazepam, and Lamotrigine, at concentration of 100 ppb (+). Total proteins were extracted from leaves harvested after 1, 2, 4, 8 and 24 h. Each set of samples was subjected to electrophoresis in five identical gels. Each gel was blotted and reacted with a different stress-specific protein antibody raised against chaperones (HSP70, HSP90, HSP100), the member of the MAPK family P38, and of the GABA-shunt enzyme glutamate decarboxylase 1 (GAD1). The photographs show the relevant region of the gel.