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. 2020 Feb 5;9(2):11. doi: 10.1038/s41389-020-0199-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Upper panel: Cells were grown under either U87EGFRwt conditioned medium (CM WT) or under U87EGFRvIII CM (CM vIII). Western blot assay was performed to examine the effect of U87EGFRvIII CM (vIII) on U87EGFRwt cells after 15 min. Bottom panel: quantification of pSrc levels normalized to GAPDH. All the proteins in the tested samples were assayed on the same membrane. The line in the middle indicates that non-relevant samples are not shown. b Representative images of immunofluorescence assays show Src activation (as represented by increase in pSrc) in U87EGFRwt cells following stimulation with CM of U87EGFRvIII (vIII). ×40 lens; scale bars represent 150 μm. c Average of pSrc intensity was quantified from eight fields (~20 cells/field). Plot is representative of at least three independent experiments, (*P = 0.005). Values are standard error (SE). d Schematic representation of Src activation in U87EGFRwt cells in response to EGFRVIII CM.