Table I.
Summary of the criteria used to identify mesenchymal stem cells as proposed by the International Society for Cellular Therapies.45
| ISCT criteria to identify MSCs |
|---|
| 1. Culture-expanded cells |
| 2. Adherence to plastic in standard culture conditions |
| 3. Phenotype positive: (> 95%) CD105, CD73, CD90 |
| 4. Phenotype negative: (≤ 2%) CD45, CD34, CD14 or CD11b, CD79a or CD19 HLA-DR |
| 5. In vitro differentiation: osteoblasts, adipocytes, chondroblasts (demonstrated by staining of in vitro cell culture)In vitro differentiation: osteoblasts, adipocytes, chondroblasts (demonstrated by staining of in vitro cell culture) |
ISCT, International Society for Cellular Therapies; MSC, mesenchymal stem cell.