Figure 4.

Analysis of mutational impact and tolerance on DNA binding in TBP (PDB: 1cdw). Shown are DROIDS image and analysis of TBP in DNA-bound and -unbound states showing (A) KL divergence colored TBP structure, (B) respective rmsf profiles, and (C) KL divergence (dFLUX) plot. Note: arrows indicate functional binding loops in the DNA minor groove, and red color indicates dampened rmsf. Shown is the maxDemon analysis (D and E) identifying conserved dynamics supporting both minor groove binding loops and connecting them through the central region of the β-sheet in the main body of TBP closest to the DNA. To see this figure in color, go online.