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. 2019 Dec 18;118(3):565–577. doi: 10.1016/j.bpj.2019.12.013

Figure 2.

Figure 2

ICAM-1 imposes strong adhesion and upstream crawling, whereas VCAM-1 allows transient adhesion and downstream crawling/rolling. (A) Shown is the adhesion strength of mobile cells, measured as the percentage of cells resistant to a shear stress of 4 dyn/cm−2 with respect to the initial number of mobile cells on the substrate. XI/YV stands for X% ICAM-1/Y% VCAM-1. (B) Shown are percentages of cells crawling upstream and rolling downstream with respect to the total number of cells migrating on the surface, under a shear stress of 4 dyn/cm−2 and for a different substrate composition. (C) Shown are rose plots of cell directions at different substrate compositions. (D) Shown is the percentage of upstream (blue) and downstream (red) crawling cells, determined here by cumulating data in the blue and red quadrant of the rose plots of cell migration for, respectively, upstream and downstream crawling cells. All data are mean ± SE; n = 6 independent experiments with at least 500 cells in each experiment. ∗p < 0.05, ∗∗p < 0.001, ∗∗∗p < 0.001 with respect to substrate composition, 100% ICAM-1/0% VCAM-1, one-way ANOVA with post hoc Dunnett’s test. To see this figure in color, go online.