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. 2020 Jan 3;21(3):1251–1257. doi: 10.3892/mmr.2020.10908

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Copyright: © Ding et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — Role of TRAF3 in birinapant-mediated apoptosis of liver cancer cells. (A) Western blotting was used to assess the protein expression levels of cIAP1 and TRAF3 in Huh7, H22 and HepG2 cells. (B) Green fluorescence (magnification, ×200) and western blotting were used to investigate the efficiency of lentivirus-mediated knockdown of TRAF3. (C) Annexin V-FITC apoptosis assays were used to assess the apoptotic rate in H22 and Huh7 cells. H22, Control vs. shRNA-TRAF3 + birinapant vs. birinapant, 12.68 vs. 21.69 vs. 32.27%; Huh7, Control vs. shRNA-TRAF3 + birinapant vs. birinapant, 13.28 vs. 25.54 vs. 36.06%. *P<0.05, **P<0.01. cIAP1, cellular inhibitor of apoptosis 1; Con, control; shRNA, short hairpin RNA; Nega, negative control; TRAF3, tumor necrosis factor receptor-associated factor 3.