Table I.
Oligonucleotide sequences of the primers used for reverse transcription-quantitative PCR analysis.
| Gene | Accession number | Primer sequence (5′-3′) | Product size (bp) |
|---|---|---|---|
| APP | P79307 | F: CATCGCTTACAAACTCGCCA | 160 |
| R: TGCCAAGAAGTCTACCCTGA | |||
| AR | NP_999479 | F: TGGTGCAATCATTTCTGCTG | 153 |
| R: ACTTTCCACCCCAGAAGACC | |||
| CCND2 | Q8WNW2 | F: ACGTCGGTGTTGGTGATCC | 154 |
| R: CCACTGACTTCAAGTTCGCC | |||
| ECE1 | HGNC:3146 | F: GTAATGTTAGCAGCGCCGTT | 110 |
| R: GAGAAGGTGCTGACGGGATA | |||
| EDNRA | NP_999394 | F: AGGTGTTCACTGAGGGCAAT | 168 |
| R: TGCCACGTCAAAATTCATGGA | |||
| EGR1 | HGNC:3238 | F: CCACTAGAACCTTCTCGTTATTCA | 103 |
| R: AGCGCCTTCAACCCTCAG | |||
| EGR2 | NP_001090957 | F: GCTGGCACCAGGGTACTG | 154 |
| R: CGGAGATGGCATGATCAAC | |||
| ESR1 | Q29040 | F: TTCCTGTCCAGGAGCAAGTT | 155 |
| R: AAGAGGGTGCCAGGATTTTT | |||
| FOS | NP_001116585 | F: CAGATCGGTGCAGAAGTCCT | 159 |
| R: ATGATGTTCTCCGGCTTCAA | |||
| GJA1 | NP_001231141 | F: GGGCAGGGATCTCTTTTGC | 150 |
| R: CAAGGTGAAAATGCGAGGGG | |||
| IHH | NP_001231399 | F: CACACGTGCCCCACTCTC | 134 |
| R: GCTTCGACTGGGTGTATTACG | |||
| IKZF2 | HGNC:13177 | F: AGGAGGTACATGGTGACTCA | 174 |
| R: CTCACAGGACACCTCAGGAC | |||
| INHBA | P03970 | F: TCAGGAAGAGCCAGATTTCG | 151 |
| R: AGGGCGGAAATGAATGAACT | |||
| INSR | HGNC:6091 | F: GTCGGTGACTCTCTCTGGAC | 160 |
| R: CACTTCTTCCGACATGTGGT | |||
| KIT | NP_001037990 | F: ACAGCCTAATCTCATCGCCA | 152 |
| R: CGCCTGGGATTTTCTCTTCG | |||
| MAP3K1 | HGNC:6848 | F: CTACATTCTTCTGCCCAGATTG | 150 |
| R: TTCAGAAAACAGTATAAAAGATGAAGA | |||
| MEF2C | NP_001038005 | F: TTTTGCTTGCATATTCTTGTTCA | 168 |
| R: CCTGGTGTAACACATCGACCT | |||
| MITF | NM_001315782 | F: CTGTTCCCGTTGCAACTTTC | 163 |
| R: CAATCACAACTTGATTGAACGAA | |||
| NFAT5 | HGNC:7774 | F: GTTGGCCTGGCTGACTTATG | 157 |
| R: GGAGGTACAATGAACCAGCTACA | |||
| NR5A1 | P79387 | F: GGTCAGCTCCACCTCCTG | 150 |
| R: GTCTTCAAGGAGCTGGAGGT | |||
| PSMB4 | NP_001231384 | F: ACGTAACCCAGGAAGCTCTC | 165 |
| R: GGTGATTGATGAGGAGCTGC | |||
| RORA | HGNC:10258 | F: CATGAGCGATCTGCTGACAT | 152 |
| R: TATGCTAGCCCCGATGTCTT | |||
| SH3D19 | HGNC:30418 | F: GCTCTGTTATTATGTCTCCAGCC | 154 |
| R: ATCTTCCCCGCAGTCTTCG | |||
| SMAD4 | Q9GKQ9 | F: TGTTTTAATTCATTTTTGTGAAGATCA | 150 |
| R: AACCACAAATGGAGCTCACC | |||
| SMARCA1 | HGNC:11097 | F: TTCCTTGGGACCTTATAGCC | 164 |
| R: AGAGAGGCATTACGTGTCAGC | |||
| VEGFA | NP_999249 | F: TCTCGATTGGACGGCAGTAG | 154 |
| R: CTCTCTTGGGTGCATTGGAG | |||
| WWTR1 | HGNC:24042 | F: ATTGATGTTCATGGGTGTGG | 152 |
| R: ATGGGGGACCATATCATTCA | |||
| ACTB | DQ845171 | F: GGGAGATCGTGCGGGACAT | 141 |
| R: CGTTGCCGATGGTGATGAC | |||
| PBGD | NM_001097412 | F: GAGAGTGCCCCTATGATGCTAT | 214 |
| R: GATGGCACTGAACTCCT | |||
| 18S rRNA | AB117609 | F: GTGAAACTGCGAATGGCTC | 105 |
| R: CCGTCGGCATGTATTAGCT |
F, forward; R, reverse.