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. 2019 Dec 31;118(3):753–764. doi: 10.1016/j.bpj.2019.12.022

Figure 1.

Figure 1

H1 contributes to multicomponent LLPS in cells. (A) Time-series confocal microscopy images, sectioned in the x-y (top) and the z-y (bottom) planes, are given, showing the coalescence of distinct H1-eGFP puncta in HeLa nuclei. (B) Two-color confocal microscopy images of H1-eGFP (left) and immunostained anti-HP1α-AlexaFluor647 (middle) in the nucleus of a HeLa cell are shown. The merged image (right) shows colocalization of H1 with HP1α (marker for heterochromatin). (C) H1 (0.3 μM, top) and H2A (1.1 μM, bottom) undergo multicomponent LLPS in vitro in presence of 150-bp DNA (0.04 μM) at physiological salt concentration (N/P ∼1). (D) The size distribution of HeLa H1 puncta, as well as H1-DNA and H2A-DNA condensates, follows a power law with an exponent of −1.5. To see this figure in color, go online.