Skip to main content
. 2019 Dec 31;118(3):753–764. doi: 10.1016/j.bpj.2019.12.022

Figure 2.

Figure 2

The NCP remains structured when partitioned into H1-mononucleosome and H2A-mononucleosome droplets. Bright-field microscopy images of (A) H1-mononucleosome and (B) H2A-mononucleosome droplets in vitro at physiological salt concentration (N/P ∼1) are shown. (C and D) Confocal microscopy images of Cy3-Cy5-labeled mononucleosomes (Cy3-DNA, Cy5-H2A) showing the partitioning of the nucleosomes into the droplets are given. (E) Cy5/Cy3 emission ratio after Cy3 excitation shows FRET emission for labeled mononucleosome in buffer solution, H1-nucleosome droplets, and H2A-nucleosome droplets, indicating that the NCP remains intact within the droplets. Error bars represent the standard deviation of nine measurements. To see this figure in color, go online.