Skip to main content
. 2019 Dec 31;118(3):753–764. doi: 10.1016/j.bpj.2019.12.022

Figure 4.

Figure 4

Phase diagrams for H1, H2A, and HP1α in the presence of DNA or polynucleosomes. A phase diagram as a function of salt concentration and DNA or polynucleosome length (expressed as number of DNA bps) (N/P ∼1) is shown for (A) H1-DNA and (B) H1-polynucleosome mixtures. (C and D) Corresponding phase diagrams for H2A-DNA and H2A-polynucleosome mixtures and (E and F) HP1α-DNA and HP1α-polynucleosome mixtures are shown. (G) Droplet surface coverage at [NaCl] = 150 mM is shown, quantified by analyzing bright-field microscopy images for H1, H2A, and HP1α droplets formed with DNA as a function of DNA length. LLPS decreases with increasing DNA length for all proteins, though to a lesser extent for H1. Droplet surface coverage at (H) [NaCl] = 150 mM and (I) [NaCl] = 300 mM for H1, H2A, and HP1α droplets formed with polynucleosomes as a function of polynucleosome length is shown. For H1, LLPS with polynucleosome is length invariant at 150 mM salt and scales favorably with length at 300 mM salt (at which spherical droplets are observed; Fig. S10) compared to H2A and HP1α. Error bars shown in G-I represent the standard deviation of analysis of ten images. To see this figure in color, go online.