Skip to main content
. 2019 Dec 31;118(3):753–764. doi: 10.1016/j.bpj.2019.12.022

Figure 5.

Figure 5

H1-polynucleosomes form liquid-like droplets at physiological salt concentration in the presence of free NTPs. H1-polynucleosomes (1400 bp) form coarse condensates in absence of ATP at physiological salt concentration at (A) N/P ∼1 and (B) N/P ∼2.4 (reduced polynucleosome concentration). (C–F) Representative bright-field and confocal images of fluorescently labeled NTPs (C ATP; D GTP; E UTP; F CTP; all at a concentration of 1.5 μm) show the presence of free nucleotides promotes H1-polynucleosome (1400 bp) LLPS into spherical, liquid-like droplets at physiological salt concentration (N/P ∼1). (G) Droplet population statistics versus droplet size (diameter, d) is given for each NTP studied, showing higher propensity for droplet formation for ATP and GTP compared to UTP or CTP. To see this figure in color, go online.