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α-carbon RMSF of the BAK1 kinase domain for each mod-form calculated from GAMD simulations, reweighted using MBAR. Phosphorylation of T450 reduces the large activation-loop fluctuations seen in the unphosphorylated state, whereas phosphorylation of T455 is insufficient for activation-loop stabilization. This figure was produced using Matplotlib 1.5 (47). To see this figure in color, go online.
