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. 2020 Jan 9;21(3):1163–1171. doi: 10.3892/mmr.2020.10922

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Copyright: © He et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 4. — Endothelial cell migration assay. RAECs (1×10⁵) were seeding on a Matrigel-coated polycarbonate membrane insert in a Transwell apparatus. Different NP cells (NP and NP-TIMP3) were also cultured with or without 100 ng/ml VEGF in the lower chamber for 24 h. For the blank control group, only medium was added into the lower chamber. The cells on the bottom surface of the insert were fixed and staining. Then the stained cells were observed and counted by a microscope. (A and B) RAEC migration was measured by Transwell assay. VEGF significantly increased the RAEC migration in the NP and NP-TIMP3 groups, while NP-TIMP3 significantly reduced migration of RAECs with or without VEGF stimulation. Data are presented as the mean ± SD. *P<0.05. Scale bar, 100 µm. RAECs, rat aorta endothelial cells; NP, nucleus pulposus; TIMP3, tissue inhibitor of metalloproteinase-3; VEGF, vascular endothelial growth factor.