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. 2020 Jan 13;21(3):1133–1144. doi: 10.3892/mmr.2020.10932

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Copyright: © Xie et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 8. — Increased apoptosis in the colon of HFD-fed mice. (A) Colon sections were stained for apoptotic cells using TUNEL assay (scale bar, 50 µm; magnification, ×40), and (B) the quantified number of apoptotic cells is shown. (C) Immunohistochemical staining for P21 expression in the colon (scale bars, 100 µm; magnification, ×40), and (D) quantified percentage of P21-positive crypt cells. (E) Representative images of immunohistochemical staining for P53 (scale bars, 100 µm; magnification, ×40), and (F) quantified percentage of P53-positive crypt cells. (G) P21 and (H) P53 mRNA expression levels in the colon. Data are presented as the mean ± standard deviation. *P<0.05 and **P<0.01, vs. Con group. HFD, high-fat diet; Con, control.