Skip to main content
. 2019 Dec 9;29(2):286–294. doi: 10.1093/hmg/ddz290

Figure 2.

Figure 2

Autophagy is induced by fenofibrate in G6pc −/− mice. (A) Western blot of LC3 and P62 with β-ACTIN loading control. Each lane represents a biological replicate (n = 4–5). (B) Quantification of LC3-II protein from A relative to β-ACTIN. (C) LC3 transcripts relative to actin. (D) P62 protein from A relative to β-ACTIN. (E) p62 transcripts relative to actin. (F) TEM images of the liver (n = 2). (Top) 5000× images for overall morphology. Scale bar = 5 μm. (Bottom) Higher magnification of autophagosomes is shown in the lower panels, including autophagosomes containing intracellular cargo and autolysosomes from the boxed area of the top panel. The autophagosome in the liver of G6pc −/− fenofibrate-treated mice also contained electron dense glycogen granules. Scale bar = 1 μm or 0.5 μm. N: nucleus, L: lipid, G: glycogen granules, AP: autophagosome, AL: autolysosome. One-way ANOVA with Tukey’s multiple comparison test was performed. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.