Table I.
Primer sequences used for the reverse transcription-quantitative PCR analysis of relative mRNA expression levels.
| Gene | Species | Primer sequence (5′→3′) |
|---|---|---|
| UII | Rat | F: GGAGGAGCTGGAGAGGACTG |
| R: GAGTCTCGGCACTGGGATCT | ||
| GPR14 | Rat | F: AATGGCTCTAGGGTCCTCCT |
| R: AACAGCCTCTGTGATGGACA | ||
| JAK2 | Rat | F: TTTGGATCCCTGGATACATACCTGA |
| R: TGGCACACACATTCCCATGA | ||
| STAT3 | Rat | F: TTTGAGACAGAGGTGTACCACCAAG |
| R: ACCACAGGATTGATGCCCAAG | ||
| MMP-2 | Rat | F: GGACAGTGACACCACGTGACAA |
| R: TTTCCAAAGTGCTGGCAGAATAGAC | ||
| TIMP-2 | Rat | F: GACACGCTTAGCATCACCCAGA |
| R: CTGTGACCCAGTCCATCCAGAG | ||
| Collagen I | Rat | F: CCCTGCTGGAGAAGAAGGAA |
| R: AGGAGAACCTTTGGGACCAG | ||
| Collagen III | Rat | F: ACTGGTGAACGTGGCTCTAA |
| R: GGACCTGGATGTCCACTTGA | ||
| β-actin | Rat | F: CAGGCATTGCTGACAGGATG |
| R: TGCTGATCCACATCTGCTGG |
UII, urotensin II; GPR14, G protein-coupled receptor 14; JAK2, Janus kinase 2; STAT3, signal transducer and activator of transcription 3; MMP-2, matrix metalloproteinase 2; TIMP-2, TIMP metallopeptidase inhibitor 2; F, forward; R, reverse.