Figure 5.

Propofol inhibits the proliferation of ovarian cancer cells through regulating the miR-374a/FOXO1 signaling axis. A2780 cells were transfected with mimics NC or miR-374a mimics and treated with propofol. (A) Cell viability was analyzed using the Cell-Counting kit-8 assay. (B) Cell cycle analysis was performed using flow cytometry. (C) Protein expression levels of FOXO1, p27 and Bim were determined and semi-quantified using western blotting. (D) Caspase-3 and caspase-9 activities were detected using colorimetric kits. Data are presented as the mean ± SD, n=3. *P<0.05 and **P<0.01 Bim, Bcl-2-like protein 11; FOXO1, forkhead box O1; miR, microRNA; NC, negative control.