Figure 3.

CD8⁺ T Cells Are More Sensitive to Suboptimal Antigens Than CD4⁺ T Cells In Vivo

CFSE-loaded LN cells from OT-I mice and B3K508 mice were injected into congenic Ly5.1 WT mice. The mice were infected with transgenic Lm expressing indicated peptides. Four days after the infection, viable splenic donor T cells (gated as CD3⁺ CD4⁺ Va2⁺ Ly5.2⁺ for B3K508 T cells and CD3⁺ CD8⁺ Va2⁺ Ly5.2⁺ for OT-I T cells) were analyzed for proliferation (CFSE) and CD25 expression by flow cytometry.

(A) Representative animals out of 6–8 per group.

(B) The percentage of donor cells among all splenic CD4⁺ or CD8⁺ T cells is shown. n = 6–8 mice in 4 independent experiments. Statistical analysis was performed using 2-tailed Mann-Whitney test.

See also Figure S3.