Figure 5.

Role of Coreceptor-LCK Coupling in Self-Reactivity of T Cell Subpopulations

(A) CD4-LCK, CD8-LCK, or CD8.4-LCK stoichiometry in LN T cells of WT and CD8.4 mice was analyzed. Mean + SEM; n = 4–9 mice from 4–8 independent experiments. Statistical analysis was performed by 2-tailed Mann-Whitney test. The CD4-LCK and CD8-LCK stoichiometries are the same as that shown in Figure 1A.

(B) Ratio of MFI levels of pTCRζ, pZAP70, and overall tyrosine phosphorylation in CD8⁺ versus CD4⁺ peripheral T cells in WT and CD8.4 chimeric mice is shown. Mean, n = 5 mice in 3 independent experiments. Statistical analysis was performed by 2-tailed Mann-Whitney test. See Figure S5D for similar data.

(C and D) LN T cells from Nur77-GFP reporter mice were analyzed by flow cytometry. The CD8⁺ CD44⁻ CD62L⁺ or CD8.4⁺ CD44⁻ CD62L⁺ T cells were analyzed for the expression of the Nur77-GFP. The percentage of Nur77-GFP⁺ and Nur77-GFP^HIGH cells is shown. Mean; n = 3–6 mice in 3–6 independent experiments. Statistical analysis was performed by 2-tailed Mann-Whitney test.

See also Figure S5.