Figure 4.

RecFOR-HR Pathway and Processing of Holliday Junction Intermediates Prevent DarT^G49D-Mediated Toxicity

(A–G) Viability of mutants following expression of darT^G49D or darT^E170A to examine the role of (A) the translesion polymerases encoded by dinB, polB, and umuD; (B) RecA; (C) RecD; (D) the RecFOR pathway; or enzymes processing Holliday junction intermediates: (E) ruvAB, (F) ruvC, and (G) recG. The recA, recF, ruvAB, ruvC, and recG mutants were complemented by providing the gene on a low copy number plasmid; n = 3 ± SD, ^∗∗∗∗p < 0.0001 by two-way ANOVA.