REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Anti-6X His tag® antibody [HIS.H8]	Abcam	Ab 18184; RRID:AB_423119
Rabbit anti-RecA polyclonal antibody	Abcam	Ab 63797; RRID:AB_1142554
Goat anti-rabbit IgG HRP conjugate secondary antibody	Santa Cruz	sc-2357
Goat anti-mouse IgG HRP conjugate secondary antibody	DAKO	Cat#P044701-2
Mouse anti-BrdU monoclonal antibody	BD Biosciences	Cat#347580, B44 clone; RRID:AB_400326
Mouse anti-ssDNA antibody	Developmental Studies Hybridoma Bank, University of Iowa	N/A
Bacterial and Virus Strains
Enteropathogenic E. coli isolate O127:H6 str. E2348/69; StrR	Knutton et al., 1987	N/A
BL21(DE3) E. coli expression strain: F-ompT hsdS(rB- mB-) dcm+ Tetrgal λ(DE3) endA [argU proL]	Agilent (previously stratagene)	Cat#200131
Dh5α (fhuA2 Δ(argF-lacZ) U169 phoA glnV44 Φ80 Δ(lacZ)M15 gyrA96 recA1 relA1 endA1 thi-1 hsdR17)	Hanahan, 1983	N/A
SM10 (thi thr leu tonA lacy supE recA::RP4-2-Tc::Mu λpir; kanR)	Simon et al., 1983	N/A
E. coli λpir (λpir ΔdapA ΔrecA; apraRermRzeoR)	Herrero et al., 1990	N/A
BTH101 (F- cya-99 araD139 galE15 galK16 rpsL1 (Strr) hsdR2 mcrA1 mcrB1)	Karimova et al., 2000	N/A
See Table S2 for additional strains generated in this study		N/A
Oligonucleotides
See Table S1 for oligonucleotides used for ADP-ribosylation assays		N/A
See Table S3 for primers used for cloning		N/A
Recombinant DNA
pCONJ4S (R6Kγ origin in a pKNG101 with sacB and sfgfp; ampR)	Kaniga et al., 1991	N/A
pBAD33 (Medium copy plasmid with an arabinose-inducible promoter; cmR)	Guzman et al., 1995	N/A
pET28A (Medium copy plasmid containing the IPTG-inducible promoter; kanR)	Novagen	Cat#69864-3
pUC19 (High copy plasmid; ampR)	NEB	Cat#N3041S
pMR101 (Low copy plasmid; kanR)	This paper	N/A
pUT18 (Vector encoding T18 fragment of B. pertussis cyaA; ampR)	Karimova et al., 1998	N/A
pKT25 (Vector encoding T25 fragment of B. pertussis cyaA; cmR)	Karimova et al., 1998	N/A
pBAD33_darTG49D (pBAD33 carrying EPEC darTG49D; cmR)	This paper	N/A
pBAD33_darTE170A (pBAD33 carrying EPEC darTE170A; cmR)	This paper	N/A
pET28A_darG (pET28A carrying EPEC darG; kanR)	This paper	N/A
pET28A_darGK54A (pET28A carrying EPEC darGK54A; kanR)	This paper	N/A
pET28A_macro (pET28A carrying EPEC macrodomain of darG (amino acids 1-113); kanR)	This paper	N/A
pET28A_macroK54A (pET28A carrying EPEC macrodomain of darGK54A (amino acids 1-113); kanR)	This paper	N/A
pET28A_Cterm (pET28A carrying EPEC C-terminal domain of darG (amino acids 1-16 + 112-330); kanR)	This paper	N/A
pMR101_recA (pMR101 carrying EPEC recA under control of its endogenous promoter; kanR)	This paper	N/A
pMR101_recF (pMR101 carrying EPEC recF under control of its endogenous promoter; kanR)	This paper	N/A
pMR101_recG (pMR101 carrying EPEC recG under control of its endogenous promoter; kanR)	This paper	N/A
pMR101_ruvAB (pMR101 carrying EPEC ruvAB under control of its endogenous promoter; kanR)	This paper	N/A
pMR101_ruvC (pMR101 carrying EPEC ruvC under control of its endogenous promoter; kanR)	This paper	N/A
pUT18_zip (pKT18 carrying leucine zipper from the yeast transcriptional activator GCN4)	Karimova et al., 1998	N/A
pUT18_darG (pKT18 carrying EPEC darG; ampR)	This paper	N/A
pUT18_macro (pKT18 carrying EPEC macrodomain of darG (amino acids 1-113); ampR)	This paper	N/A
pUT18_Cterm (pKT18 carrying EPEC C-terminal domain of darG (amino acids 1-16 + 112-330); ampR)	This paper	N/A
pKT25_darTG49D (pKT25 carrying EPEC darTG49D; ampR)	This paper	N/A
pKT25_zip (pKT25 carrying leucine zipper derived from the yeast transcriptional activator GCN4)	Karimova et al., 1998	N/A
Software and Algorithms
ImageJ	Schneider et al., 2012	https://imagej.nih.gov/ij/
Prism - GraphPad	GraphPad Software, Inc. Accessed 5 November 2008	https://www.graphpad.com/scientific-software/prism/
MATLAB	MATLAB, 2013; MATLAB, 2016	https://www.mathworks.com/products/matlab.html