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. 2019 Feb 26;26(9):2298–2306.e5. doi: 10.1016/j.celrep.2019.02.006

Figure 3.

Figure 3

Knock Down of GLT8D1 and Overexpression of Mutant GLT8D1 Produce Toxicity and Motor Impairment in Zebrafish Embryos

(A, B, and D) Mean distance moved (mm) during three light-dark cycles at 5 days post-fertilization is reduced following injection with 1.5 ng GLT8D1 splice blocking morpholino (A) compared to 1.5-ng control morpholino (n = 160 from 3 clutches); and following injection with 1-nl mature mRNA encoding human GLT8D1-R92C (B) (n = 96 from 2 clutches) or GLT8D1-G78W (D) (n = 48 from one clutch) compared to 1-nl mature mRNA encoding wild-type human GLT8D1.

(C) Injection of 1-nl mature mRNA encoding human GLT8D1-R92C significantly reduces survival of zebrafish embryos at 24 hours and 48 hours compared to mature mRNA encoding wild-type human GLT8D1. WT, wild-type; SB, splice blocking morpholino. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001.

Error bars represent SEM (A, B, and D) or SD (C).