Figure 1.

Features of the SNP-CRISPR user interface (UI). Users select the species of interest, enter an E-mail address, upload variant information including the genome coordinates and sequence changes, choose to target nearby variants individually or together, and then submit the job. Usually within half an hour, an E-mail is sent automatically to the user with a link to a results page that displays the designs for wild type as well as mutant alleles, side by side with calculated scores. The mutant base(s) are shown in lower case and the wild type sequence in upper case.