Figure 2.

Images of transgenic strains. A. Images of TOMM-20::HaloTag labeled with JF₆₄₆ HaloTag ligand in the adult gonad (outlined with curved dotted line), including an inset of the region in the stippled box. B. Images of embryos expressing TOMM-20::HaloTag labeled with JF₆₄₆ HaloTag ligand at the 1-cell, 4 cell and ∼100 cell stages. C. Images of a 4 cell embryo expressing TOMM-20::HaloTag labeled with JF₆₄₆ HaloTag ligand (magenta) and COX-4::GFP (green) (Raiders et al., 2018). D – F. Images of embryos expressing the indicated transgenes at the 4-cell stage. G – I. Images of a 4 cell embryo expressing TOMM-20::Dendra2 before and after photoconversion (PC). Dendra2 switches from green to red fluorescence upon photoconversion. J – M. Images of embryos expressing the indicated transgenes at the 1-cell, 4 cell and ∼100 cell stages.