Table 3. Primers used in this study.
| Name | Description | Sequence (SAP1 site and Overhang) | Corresponding plasmid |
|---|---|---|---|
| XF32F | mex-5 promoter (F) | GCAGCTCTTCGTGGATATCAGTTTTTAAAAAATTA | pXF121 |
| XF32R | mex-5 promoter (R) | GCAGCTCTTCGCATTCTCTGTCTGAAACA | |
| JF5F | tomm-20 (F) | GCAGCTCTTCGATGTCGGACACAATTCTTGG | pJF7 |
| JF5R | tomm-20 (R) | GCAGCTCTTCGACCCTCCAAGTCGTCGGTGTC | |
| JF1F | gfp (F) | GCAGCTCTTCGATGTCCAAGGTAACACTTAGTTT | pJF5 |
| JF1R | gfp (R) | GCAGCTCTTCGACCGCCGCTTCCCTTGTAGAGCTCGTCCAT | |
| JF2F | gfp (F) | GCAGCTCTTCGGGTGGAAGCGGCTCCAAGAACACTTAGTTT | pJF6 |
| JF2R | gfp (R) | GCAGCTCTTCGTTACTTGTAGAGCTCGTCCAT | |
| XF17F | hsp-3 (1-19aa) (F) | ATGAAGACCTTATTCTTGTTGGGCTTGATCGCCCTATCCGCCGTCAGTGTCTACTGC | |
| XF17R | hsp-3 (1-19aa) (R) | ACCGCAGTAGACACTGACGGCGGATAGGGCGATCAAGCCCAACAAGAATAAGGTCTT | |
| spe-11(SAP C1) F | spe-11 promoter (F) | GCAGCTCTTCGTGGGTCGACAGAACATTTTTCCGT | pSDH60 |
| spe-11(SAP C1) R | spe-11 promoter (R) | GCAGCTCTTCGCATTTTATCTAGTCGGTTTGCGA | |
| XF24F | halotag (F) | GCAGCTCTTCGATGGCCGAGGTAACACTTAGTTTTTGT | pXF89 |
| XF24R | halotag (R) | GCAGCTCTTCGACCGCCGCTTCCTCCGGAGATCTCGAGGGT | |
| XF63F | mkate2 (F) | GCAGCTCTTCGATGGTCTCCGAGCTCATTAAAGAAAACA | pXF222 |
| XF63R | mkate2 (R) | GCAGCTCTTCGACCACCTCCACCTCCACGGTGTCCGAGCTTGG | |
| ePDZ (SAP C2) F | epdz (F) | GCAGCTCTTCGATGCCAGAGCTCGGATTCTCGAT | pSDH61 |
| ePDZ (SAP C2) R | epdz (R) | GCAGCTCTTCGACCAGCTCCCGTCGCGACGGGTGGATCAC | |
| XF79F | cox-4 (F) | GCAGCTCTTCGATGATGCTGCCACGTTTG | pXF262 |
| XF79R | cox-4 (R) | GCAGCTCTTCGACCCTTCCACTTCTTGTTCTCGTAATC | |
| XF76F | npp-20 (F) | GCAGCTCTTCGATGACCACGGTCCGCCAG | pXF250 |
| XF76R | npp-20 (R) | GCAGCTCTTCGACCTCTCTGAGCTCCCGGAGCT | |
| XF23F | halotag (F) | GCAGCTCTTCGGGTGGAAGCGGCGCCGAGGTAACACTTAGTTTTTGT | pXF88 |
| XF23R | halotag (R) | GCAGCTCTTCGTTATCCGGAGATCTCGAGGGT | |
| XF53F | mkate2 (F) | GCAGCTCTTCGGGTGGAGGTGGAGGTGTCTCCGAGCTCATTAAAGAAAAC | pXF130 |
| XF53R | mkate2 (R) | GCAGCTCTTCGTTAACGGTGTCCGAGCTTGGA | |
| XF22F | halotag::hdel (F) | GCAGCTCTTCGGGTGGAAGCGGCGCCGAGGTAACACTTAGTTTTTGT | pXF90 |
| XF22R | halotag::hdel (R) | GCAGCTCTTCGTTAGAGTTCGTCATGTCCGGAGATCTCGAGGGT | |
| SIM8F | mscarlet (F) | GCAGCTCTTCGATGGTCTCCAAGGGCGAGGCA | pSM10 |
| SIM8R | mscarlet (R) | GCAGCTCTTCGACCACCTCCACCTCCCTTGTACAGCTCGTCCATTCCT | |
| SIM10F | dendra2 (F) | GCAGCTCTTCGATGAACCTTATTAAGGAAGATATG | pSM12 |
| SIM10R | dendra2 (R) | GCAGCTCTTCGACCGCCGCTTCCCCATACTTGACTTGGTAG | |
| SIM1F | dendra2 (F) | GCAGCTCTTCGGGTGGAAGCGGCAACCTTATTAAGGAAGATATG | pSM03 |
| SIM1R | dendra2 (R) | GCAGCTCTTCGTTACCATACTTGACTTGGTAG | |
| SIM2F | mkate2 (F) | GCAGCTCTTCGGGTGGAGGTGGAGGTGTCTCCGAGCTCATTAAAGAAAACA | pSM04 |
| SIM2R | mkate2 (R) | GCAGCTCTTCGCGTACCTCCACCTCCACGGTGTCCGAGCTTGGA | |
| SIM3F | mkate2 (F) | GCAGCTCTTCGACGGGAGGTGGAGGTGTCTCCGAGCTCATTAAAGAAAACA | pSM05 |
| SIM3R | mkate2 (R) | GCAGCTCTTCGTTAACGGTGTCCGAGCTTGGA | |
| SIM6F | mscarlet (F) | GCAGCTCTTCGGGTGGAGGTGGAGGTGTCTCCAAGGGCGAGGCA | pSM08 |
| SIM6R | mscarlet (R) | GCAGCTCTTCGTTACTTGTACAGCTCGTCCATTCCT | |
| mScarlet (SAPC3)F | mscarlet (F) | GCAGCTCTTCGGGTGTCTCCAAGGGCGAGGCAGTCAT | pSDH57 |
| mScarlet (SAPC3)R | mscarlet (R) | GCAGCTCTTCGCGTGGCCGCGGCTTTTGCAGCGG | |
| XF84F | lov (F) | GCAGCTCTTCGACGCCTCGTCTTGCTGCT | pXF276 |
| XF84R | lov (R) | GCAGCTCTTCGTTAGACCCAAGTGTCGACGGC | |
| XF12F | pie-1 3′UTR (F) | GCAGCTCTTCGTAATTTTGCCGTATTTTCCAT | pXF85 |
| XF12R | pie-1 3′UTR (R) | GCAGCTCTTCGTACATCATCGTTCACTTTTCAC | |
| tbb2 3′UTR (SAPC5)F | tbb-2 3′UTR (F) | GCAGCTCTTCGTAAATGCAAGATCCTTTCAAGCATTC | pSDH54 |
| tbb2 3′UTR (SAPC5)R | tbb-2 3′UTR (R) | GCAGCTCTTCGTACGACTTTTTTCTTGGCGGCAC | |
| Halo (SAP C3)F | halotag (F) | GCAGCTCTTCGGGTGGAAGC | pSDH51 |
| Halo (SAP C3)R | halotag (R) | GCAGCTCTTCGCGTTCCGGAGATCTCGAGGGTGG | |
| ePDZ (SAP C4)F | epdz (F) | GCAGCTCTTCGACGGGAGGTTCCGGAGGATCTGGC | pSDH52 |
| ePDZ (SAP C4)R | epdz (R) | GCAGCTCTTCGTTACGTCGCGACGGGTGGAT | |
| unc-54 (SAPC5)F | unc-54 3′UTR (F) | GCAGCTGTTCGTAAGAGCTCCGCATCGGCCGCTG | pSDH66 |
| unc-54 (SAPC5)R | unc-54 3′UTR (R) | GCAGCTCTTCGTACAAACAGTTATGTTTGGTATATTGGGA | |
| Eg717 | Replace pCFJ350 MCS (F) | TCGAGTGGCGAAGAGCCCATGGATCCCATATGGAATTCTGCAGGCCTGCTCTTCGGTAA | pXF87 |
| Eg718 | Replace pCFJ350 MCS (R) | CTAGTTACCGAAGAGCAGGCCTGCAGAATTCCATATGGGATCCATGGGCTCTTCGCCAC | |
| XF30F | Mutate SapI site in pCFJ350 | GATTATGGGCACTTCTTTTATCC | pXF87 |
| XF30R | Mutate SapI site in pCFJ350 | CGACAAGCAACTTTTCTATAC | |
| XF31F | Mutate SapI site in pCFJ350 | AATGGCGAAGtGCAAAGCAGAG | pXF87 |
| XF31R | Mutate SapI site in pCFJ350 | GTTTCCTGAAAATAATGTAACTTGAATTG |
Note: For the expression plasmid pJF13 annealed oligos were used to generate HSP-3(aa 1-19) in cassette 2.
Additional oligo sequences used to generate pSDH50:
TOMM-20 short forward. GCAGCTCTTCGATGTCGGACACAATTCTTGGTTTCAAcaaatcaaacgtcgttttggctgctggaattgctggagccgctttcctcggctactgcatttacttcgatcataagagaatcaacgctccagactacaaggacaagattaggcaaagtcagtgttttaacaacatatttccttcggatttttatctaaaaacaacttattttctttcagagagaCGTGCCCAGGCTGGAGCAggagctggtgcaggcgctggagccggagccGGTCGAAGAGCtgc.
TOMM-20 short reverse GCAGCTCTTCGACCggctccggctccagcgcctgcaccagctccTGCTCCAGCCTGGGCACGtctctctgaaagaaaataagttgtttttagataaaaatccgaaggaaatatgttgttaaaacactgactttgcctaatcttgtccttgtagtctggagcgttgattctcttatgatcgaagtaaatgcagtagccgaggaaagcggctccagcaattccagcagccaaaacgacgtttgatttgTTGAAACCAAGAATTGTGTCCGACATCGAAGAGCtgc.