. 2019 Dec 17;10(2):635–644. doi: 10.1534/g3.119.400822

Table 4. MosSCI targeting vectors used in this study.

Name	Comments	Assembly
pXF87	MosSCI backbone	Derived from pCFJ350
		Donor vectors used for assembly								Assembly efficiency
		Cassettes								Digestion	Sequencing
		1		2		3		4
pJF13	ER lumen, Halotag	pXF121		XF17F/R^a		pXF90		pXF85		4/5	2/2
pJF17	Mitochondrial OM, Halotag	pXF121		pJF7		pXF88		pXF85		4/5	1/2
pXF253	ERES + nuclear pores (NPP-20), GFP	pXF121		pXF 250		pJF6		pXF85		4/6	2/2
pXF255	ERES + nuclear pores (NPP-20), Halotag	pXF121		pXF 250		pXF88		pXF85		5/6	2/2
pXF266	Mitochondrial matrix, Halotag	pXF121		pXF 262		pXF88		pXF85		1/4	1/1
pSM20	Mitochondrial OM, mKate2	pXF121		pJF7		pXF130		pXF85		4/5	2/2
pSM22	Mitochondrial OM, mScarlet	pXF121		pJF7		pSM08		pXF85		4/5	2/2
pSM17	Mitochondrial OM, Dendra2	pXF121		pJF7		pSM03		pXF85		4/5	2/2
pSM16	Mitochondrial OM, GFP	pXF121		pJF7		pJF6		pXF85		2/5	2/2
		1	2		3A		3B		4
pSDH68	Mitochondrial OM, Halotag, LOV	pXF121	pSDH50		pSDH51		PCR fragment		pSDH54	11/15	2/2

Annealed oligos.