Table 1.
Antibody sources, manufacturers, dilutions, clones, types, incubation times, and antigen retrieval methods.
| Antibody | Antibody source | Dilution | Clone | Type | Incubation time | Antigen retrieval |
|---|---|---|---|---|---|---|
| GATA3 | Cell Marque | 1:250 | L50-823 | Monoclonal mouse | 30 min | HIER, trilogy |
| ERα | Leica | Ready to use | 6 F11 | Monoclonal mouse | 60 min | HIER, citrate pH 6 |
| PR | Leica | Ready to use | 16 | Monoclonal mouse | 30 min | HIER, citrate pH 6 |
| HER2/neu | Ventana Medical System | 1:15 | 4B5 | Monoclonal rabbit | 30 min | HIER, TR6 |
| CK5/6 | Dako | 1:100 | D5/16 B4 | Monoclonal mouse | 45 min | HIER, EnVision FLEX target retrieval solution, high pH |
HIER = heat-induced epitope retrieval. Previously tested mammary gland samples were used as positive controls; negative controls were obtained by replacement of the primary antibody by IgG. Detection system used was NovoLink detection system (Leica Biosystems, Buffalo Grove, IL).