Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 5;17:50. doi: 10.1186/s12974-020-1712-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2020

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 1 — Isolation of primary astrocytes and experimental design. a Representative images of isolated primary astrocytes. Green, GFAP; blue, nuclei; bar size, 100 μm. b Illustration of experimental design and conditioned media. A/Lps, conditioned medium of astrocytes cultured with LPS; A/MC, conditioned medium of astrocytes cultured with M/C; A/MLps, conditioned medium of astrocytes cultured with M/Lps; GFAP, glial fibrillary acidic protein; LPS, lipopolysaccharide; M/C, microglia conditioned medium without LPS stimulation; M/Lps, microglia conditioned medium pre-activated with LPS