Fig. 10.

Effect of paroxetine on neurotrophic responses of astrocytes. Cells were pretreated with paroxetine at different concentrations for 30 min followed by incubation with microglia conditioned media for 24 h. a Quantitative PCR analyses of BDNF and MANF expression. The mRNA levels were expressed relative to the one incubated with M/Lps alone, which was set as 100. b Concentrations of BDNF in culture media. Values are means ± SE, n = 3 for a, n = 4 for b. Statistical comparisons were performed using one-way ANOVA, followed by Tukey’s post hoc test. Different letters indicate p < 0.05. BDNF, brain-derived neurotrophic factor; MANF, mesencephalic astrocyte-derived neurotrophic factor; M/C, microglia conditioned medium without LPS stimulation; M/Lps, microglia conditioned medium pre-activated with LPS