Fig. 6.

Effect of paroxetine on M/Lps-induced p65/NF-κB nuclear translocation in astrocytes. Cells were pretreated with or without 10 μM of paroxetine for 30 min followed by stimulation with M/Lps for 30 min. a Representative images of p65 immunostaining. Green, GFAP; red, p65; blue, nuclei; bar size, 200 μm. b Fluorescence intensity of nuclear p65 was determined by quantifying 15 cells in multiple random fields, followed by normalization to their respective nuclei staining. Data are expressed as means ± SE from three independent experiments. Statistical comparisons were performed using two-way ANOVA. Different letters and * indicate p < 0.05. GFAP, glial fibrillary acidic protein; M/Lps, microglia conditioned medium pre-activated with LPS