Figure 4.

Cyclic expression of C. gigas core clock genes in controlled conditions. Temporal profiles of clock gene mRNA levels were measured in C. gigas gills sampled in the laboratory (a) under constant darkness (DD) and (b) LD alternating regimen 9 : 15 h. Grey areas indicate darkness. Values are expressed as relative mRNA levels, means ± s.e.m., n = 9 oysters per time point in the DD and n = 5–6 oysters per time point in LD. For clock genes full names, see the electronic supplementary material, table S3. The blue CT indicates significant circatidal rhythm (approx. 12.4 h), and the red CD/D indicates significant circadian/daily rhythm (approx. 24 h). Asterisks indicate the power of significance: *p < 0.05; **p < 0.01; ***p < 0.001. (c) Inserted table of exact adjusted p-values using RAIN analysis for DD and LD experimental data.