Table 1.

Summary of culture process set points and controls

Culture parameter	Setpoint or description
Culture volume	4 L
Sparge rate	0–30 ccm
Dissolved oxygen	50%
Dissolved oxygen control	Air, O2, and CO2
Agitation rate	150 rpm
Temperature	37°C
Inoculation density	3 × 105–1 × 106 cells/ml
pH	7.1
pH control	0.5 M NaOH and CO2