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. 2020 Feb 5;6:2. doi: 10.1186/s40851-020-0154-y

Table 2.

Primers and references for PCR protocols for amplification of the four DNA fragments sequenced in the study

DNA marker Primer name Primer direction Primer sequence (5′-3′) Primer source PCR programme
18S rRNA 18S_Tar_Ff1 forward AGGCGAAACCGCGAATGGCTC [57] [58]
18S_Tar_Rr1 reverse GCCGCAGGCTCCACTCCTGG
28S rRNA 28S_Eutar_F forward ACCCGCTGAACTTAAGCATAT [59, 60] [60]
28SR0990 reverse CCTTGGTCCGTGTTTCAAGAC
ITS-2 ITS2_Eutar_Ff forward CGTAACGTGAATTGCAGGAC [7] [7]
ITS2_Eutar_Rr reverse TCCTCCGCTTATTGATATGC
COI COI_Para_F forward TTTCAACAAACCACAAAGATATYGG [61] [9]
COI_Eutar_Rr reverse TAAACTTCTGGGTGACCRAARAAYCA
LCO1490 forward GGTCAACAAATCATAAAGATATTGG [62]
COI_Mac_Rr reverse TGTTGGTATARAATWGGGTC [101]