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Simultaneous detection of ILC1s, ILC2s, and ILC3s in a single section by the sequential quadruple staining technique. Identification is based on the expression of archetypical transcription factors T-bet (green), GATA3 (blue), and RORγt (red) to identify ILC1s, ILC2s, and ILC3s, respectively, and lack of expression of lineage markers CD3/CD20/CD79α (black). Scale bar: 10 μm. Abbreviations: ILC, innate lymphoid cell.
