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. 2020 Jan 30;8:15. doi: 10.3389/fcell.2020.00015

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Copyright © 2020 Ma, Pan, Liu, Liu, Xiang, Pan, Zhang and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Agrin participates in regulating nerve sprouting after BoNT/A application. (A) Western blot analysis of agrin at NMJs after BoNT/A injection. GAPDH was used as internal control. (B) Quantitation of agrin protein was performed using GeneTools from SynGene software and normalized to GAPDH. *P < 0.05 versus control. (C) α-Bungarotoxin staining analysis of the number of AChRs after BoNT/A and agrin-Ab injection at week 1. Nuclei were counterstained with DAPI (4′-6-diamidino-2-phenylindole; blue). Images were merged as indicated. Scale bar, 100 μm. (D) Number of AChRs; bars represent mean ± SD of three different experiments. *P < 0.05, **P < 0.01 compared to the control group. #P < 0.05 compared to the BoNT/A group.