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. 2020 Jan 30;8:15. doi: 10.3389/fcell.2020.00015

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Copyright © 2020 Ma, Pan, Liu, Liu, Xiang, Pan, Zhang and Jin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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miR-144-agrin signaling participates in regulating NMJ formation. (A) Real time RT-PCR analysis of miR-144 expression after BoNT/A injection. *P < 0.05, compared with the control group. Data represent mean ± SD of three different experiments. (B) The relative trends of miR-144 and agrin expression (BoNT/A group/Control group). (C) The relative expression level of agrin from rats 1, 4, and 8 weeks after birth analyzed by western blot. GAPDH was used as an internal control. (D) Protein levels were normalized to those of GAPDH. *P < 0.05, compared to the control (8W) group. (E) The relative expression level of miR-144 from rats 1, 4, and 8 weeks after birth analyzed by real time RT-PCR assay. GAPDH was used as an internal control. *P < 0.05, compared to the control (8W) group. (F) The relative trends of miR-144 and agrin expression (BoNT/A group/Control group).