Figure 2. The effect of TMF on anxiety behavioral outcomes was investigated by open-field test (OFT). (A) The tracking for the OFT by the SMART program. (B) The time spent in central area of the open-field box. (C) The time spent in periphery of the open-field box. (D) The frequency of rearing behavior in 5 min. (E) The frequency of grooming behavior in 5 min. The data are the mean ± SEM from three independent experiments (**p<0.01, ***p<0.001 compared with the control group, ^#p<0.05, ^##p<0.01 compared with the DEX group).